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Recombinant human alpha-Synuclein (Delta-NAC) protein

A deletion mutant of alpha-synuclein that lacks the NAC region (amino acid 61-95) . alpha-synuclein (deltaNAC) was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques. 6 amino acids were added as a linker.

Product Specifications

Product Name Alternative

SNCA, NACP, PARK1, PARK4, PD1, α-synuclein Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease 4, autosomal dominant Lewy body

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by BCA assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

11.9 kDa (111aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

A-Synuclein (Delta-NAC), SNCA, NACP, PARK1, alpha-Synuclein Delta-NAC, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, Alpha-synuclein, Alpha synuclein, Alpha-synuclein, isoform NACP140, alphaSYN, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid precursor, Non-A-beta component of alzheimers disease amyloid, precursor of, PARK 1, PARK 4, PARK4, Parkinson disease familial 1, PD 1, PD1, Snca synuclein, alpha (non A4 component of amyloid precursor), Synuclein alpha, Synuclein, alpha (non A4 component of amyloid precursor), SNA2008-10 µg, SNA2008-20 µg, SNA2008-50 µg, SNA2008-100 µg, SNA2008-250 µg, SNA2008-500 µg, SNA2008-1 mg, SNA2008-10, SNA2008-20, SNA2008-50, SNA2008-100, SNA2008-250, SNA2008-500, SNA2008-1

References & Citations

Ueda., et al. (1993) Proc. Natl.Acad.Sci. 90, 11282-11286.

Other References

; ; Kawakami F, et al. Stimulatory effect of α-synuclein on the tau-phosphorylation by GSK-3β. (FEBS J. 2011) {https://pubmed.ncbi.nlm.nih.gov/21985244/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_000336.1

Uniprot Accession Number

P37840

Species

Human

AA Sequence

MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK GTEIWMKKDQ LGKNEEGAPQ EGILEDMPVD PDNEAYEMPS EEGYQDYEPE A

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