Human Beta-2-Microglobulin, B2M ELISA Kit

B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells) . The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV.

Concentration | O.D. | Average | Corrected ------------------------------------------ 200 pg/ml | 2.185 | 2.226 | 2.108 2.267 100 pg/ml | 1.271 | 1.263 | 1.145 1.255 50 pg/ml | 0.711 | 0.702 | 0.584 0.693 25 pg/ml | 0.426 | 0.409 | 0.291 0.392 12.5 pg/ml | 0.278 | 0.26 | 0.142 0.242 6.25 pg/ml | 0.187 | 0.185 | 0.067 0.183 3.125 pg/ml | 0.154 | 0.16 | 0.042 0.166 0 pg/ml | 0.11 | 0.118 | 0 0.126

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 12.8 | 0.82 | 6.4 Intra-assay | 2 | 20 | 53.4 | 3.5 | 6.5 Intra-assay | 3 | 20 | 144 | 7.1 | 4.9 Inter-assay | 1 | 20 | 12.7 | 0.98 | 7.7 Inter-assay | 2 | 20 | 52.9 | 3.4 | 6.4 Inter-assay | 3 | 20 | 142 | 10.6 | 7.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail