Purified Anti-Human IL-6 Antibody[MQ2-13A5], Functional Grade

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130) . IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells. None (Azide-Free, Low Endotoxin) are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays blocking, neutralizing, activation or depletion where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.

B cell differentiation factor; B cell stimulatory factor 2; BSF 2; Hepatocyte stimulatory factor; Hybridoma growth factor Interferon beta-2; IL 6; Interferon beta 2; Interleukin 6 (interferon beta 2) ; Interleukin BSF 2; Interleukin-6; IL-6; B-cell hybridoma growth factor; Interleukin HP-1; IFNB; Interleukin HP; Interferon Beta; B-Cell Stimulatory Factor; IFN-Beta; B-cell hybridoma growth factor; B-Cell Stimulatory Factor 2; BSF2; BSF-2; CDF; CTL Differentiation Factor; HGF; HSF; Hybridoma Growth Factor; IFN-Beta-2; IL-6; Interferon Beta-2; Interleukin 6; Interleukin HP-1; Interleukin-6; IL6; IFNB2

Immunology; Cancer; Metabolism; Neuroscience; Invasion and Metastasis; Cytokine and Growth Factors; Metabolic disorders; Obesity; Jak/STAT Signaling Pathway; Neurodegenerative Diseases Markers; Angiogenesis; Angiogenic growth factors; Tumor Immunity; Prostate Cancer Markers; Tumor Biomarkers

Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.