Ku 70 Recombinant Rabbit Monoclonal Antibody [JM61-31]
The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70 (p70) and Ku-86 (p86) which is also known by the synonym Ku-80 or (p80). Ku was first described as an autoantigen to which antibodies were produced in a patient with scleroderma polymyositis overlap syndrome, and was later found in the sera of patients with other rheumatic diseases. Both subunits of the Ku protein have been cloned, and a number of functions have been proposed for Ku, including cell signaling, DNA replication and transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of its DNA binding activity, serving as the regulatory component of the DNA-associated protein kinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activate transcription from the U1 small nuclear RNA and the human transferrin receptor gene promoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF), composed of two subunits, has been identified as a positive regulator of RNA polymerase I transcription initiation.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
5'-dRP lyase Ku70, CTC75, CTCBF, Ku70, TLAA, XRCC6
Swiss Prot
P12956 Human
Cellular Locus
Nucleus. Chromosome.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide within Human Ku70 aa 521-564 / 609.
Isotype
IgG
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IP, IF-Cell, IF-Tissue, IHC-P, FC
Positive Control
HeLa cell lysate, Jurkat cell lysate, HeLa, human tonsil tissue.
Concentration
1 mg/mL
Dilution
WB: 1:500-1:2,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000 ;FC: 1:1,000 ;IP: Use at an assay dependent concentration.
Purity
Protein A affinity purified.
Form
Liquid
Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 70 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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