ROS-ID®NO Detection kit

The ROS-ID ® NO detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

A sample from each lot of ROS-ID ® NO Detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard red (650/670 nm) fluorescent cubes. The following results are obtained: Nitric Oxide positive control samples induced with L-Arginine exhibit red fluorescence with a red punctuate cytoplasmic staining pattern. Cells incubated with the NO Scavenger (c-PTIO), post induced with L-Arginine don’t demonstrate any red fluorescence.