ROS-ID® Superoxide detection kit

With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage.

The ROS-ID ® Superoxide detection kit is a member of the CELLESTIAL ® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL ® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.

A sample from each lot of ROS-ID ® Superoxide detection kit is used to stain HeLa cells using the procedures described in the user manual. The stained cells are analyzed using a wide-field fluorescence microscope equipped with standard orange (e.g., 550/620 nm) fluorescent cube. The following results are obtained: ROS positive control samples induced with Pyocyanin exhibit bright orange fluorescence in the nucleus. Cells pretreated with the ROS inhibitor don’t demonstrate any orange fluorescence signal upon induction.

Superoxide Detection Reagent (Orange), 300 nmoles ROS Inducer (Pyocyanin), 1 μmole ROS Inhibitor (N-acetyl-L-cysteine), 2 x 10 mg Wash Buffer Salts, 1 pack