P300 (catalytic domain) (human), (recombinant)
Product Specifications
Specifications
≥250 pmol/min/μg assayed as production of CoA-SH from AcCoA in the presence of a peptide comprising human p53 residues 368-386 (Prod. No. BML-P198) . CoA-SH is determined colorimetrically by reaction with DTNB (5,5′-Dithiobis (2-nitrobenzoic acid) ) .
Product Name Alternative
E1A binding protein p300, Histone acetyltransferase p300
Certification
RUO
UniProt
Q09472
Source
Produced in E. coli . Catalytic domain (aa 1284-1673) of human p300.
Purification
Partially purified by single-step affinity chromatography and gel filtration.
Molecular Weight
45.1 kDa
Shipping Conditions
Dry Ice
Storage Conditions
-80°C
Formulation
Liquid. In 50mM TRIS/HCl, pH 8.0, containing 0.1mM EDTA, 10% glycerol.
Applications Notes
Can be used to study p300 kinetics and regulation, for screening for inhibitors or activators and for radiolabeling of proteins or peptides with, for example, [ 3 H]-Acetyl CoA.
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