Matrix metalloproteinase (MMP) multipack-2

Note: MMP-3 is unique in that its pH optimum is 6.0. Activity of this enzyme in pH 7.0 buffers is significantly reduced. Note: Human (but not mouse) MMP-11 has very unique substrate preferences, and only poorly cleaves typical MMP substrates. Use much higher substrate concentrations and longer incubation times.

Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl 2 , 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.

Contains 10μg of each enzyme, provided in a screw-cap microfuge tube. MMP-3 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE109), MMP-7 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE181), MMP-10 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE329), MMP-11 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE282), MMP-12 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE138) .