Anti-HER2 (Phospho-Tyr877) Antibody

Rabbit Polyclonal Antibody specific to HER2 (Phospho-Tyr877)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-HER2 (Phospho-Tyr877) Rabbit Polyclonal Antibody

Gene Name

ERBB2

Gene ID

164870

NCBI Gene ID

UniProt

P04626

Cellular Locus

[Isoform 1]: Cell membrane, Cytoplasm, perinuclear region. Nucleus. Note=Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of tyrosine 877 (T-E-Y(p)-H-A) derived from human HER2 and conjugated to KLH.

Target Antigen

Receptor tyrosine-protein kinase erbB-2

Target

HER2 (Phospho-Tyr877)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. {PubMed:10358079, PubMed:15380516, PubMed:21555369}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~185kDa is detected. Immunofluorescence: use at dilution of 1:100-1:200. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat HER2 only when phosphorylated at tyrosine 877.Accession no.: P04626, NP_001005862.1

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years HER2 has become an important biomarker and target of therapy for approximately 30% of breast cancer patients.