Recombinant SARS-CoV2 S-Protein ACE2 Binding Domain
The sequence below is that of our recombinant construct of the SARS-CoV-2 spike or S-protein which includes the entire region which interacts with ACE2. The specific binding to ACE2 is essential for viral internalization and infection. We designed this construct based on amino acids 308-541 in the S-protein sequence in Isolate Wuhan-Hu-1, complete genome. This is a defined globular domain recently shown to include all of the amino acids necessary for ACE2 binding. The construct was expressed in and purified from E. coli and includes an N-terminal His-tag and other vector derived sequence shown underlined below.
Product Specifications
Applications
ELISA
Components
Supplied as 1mg/mL in 6M urea, 10mM phosphate buffer pH=7.5
Shipping Conditions
Stable at 4°C for several months. For longer term store at -20°C, minimize freeze/thaw cycles
Storage Conditions
Stable at 4°C for several months. For longer term store at -20°C, minimize freeze/thaw cycles
Applications Notes
ELISA
Amino Acids
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