CCM2 Recombinant Protein

Source : Escherichia Coli. CCM2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 311 amino acids (66-353 a.a) and having a molecular mass of 34.3kDa.CCM2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Cerebral Cavernous Malformation 2, also known as CCM2 is a piece of the CCM signaling pathway which is a vital regulator of heart and vessel formation as well as integrity. CCM2 performs through the stabilization of endothelial cell junctions. In addition, CCM2 functions as a scaffold protein for MAP2K3-MAP3K3 signaling. CCM2 plays a key role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock. Mutations in CCM2 result in cerebral cavernous malformations. Multiple transcript variants encoding dissimilar isoforms have been discovered for CCM2.

Product Specifications

Product Name Alternative

Cerebral Cavernous Malformation 2||C7orf22||malcavernin||Cerebral Cavernous Malformations 2 Protein||Chromosome 7 Open Reading Frame 22||OSM||MGC4067.

Purification

"Greater than 90.0% as determined by SDS-PAGE."

Components

CCM2 protein solution (0.5mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please avoid freeze thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSEVKYLGQ LTSIPGYLNP SSRTEILHFI DNAKRAHQLP GHLTQEHDAVLSLSAYNVKL AWRDGEDIIL RVPIHDIAAV SYVRDDAAHL VVLKTDDSST KVDIKETYEV EASTFCFPESVDVGGASPHS KTISESELSA SATELLQDYM LTLRTKLSSQ EIQQFAALLH EYRNGASIHE FCINLRQLYG DSRKFLLLGL RPFIPEKDSQHFENFLETIG VKDGRGIITD SFGRHRRALS TTSSSTTNGN RATGSSDDRS APSEGDEWDR MISDISSDIEALGCSMDQDS A