IGF1 Des1-3 Recombinant Protein

Source : Escherichia Coli. IGF-I Des (1-3) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7372 Dalton. IGF-1 Des1-3 is purified by proprietary chromatographic techniques. The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250) . Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor, ' which later became known as 'somatomedin' (Daughaday et al., 1972) . Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003) .

Product Specifications

Product Name Alternative

Somatomedin C||IGF-I||IGFI||IGF1||IGF-IA||Mechano growth factor||MGF||Des (1-3) ||Des1-3||Des 1-3||Des (1-3) ||IGF-1 (4-70) .

Purification

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Components

The protein was lyophilized after extensive dialysis against 50mM acetic acid buffer.

Storage Conditions

Lyophilized IGF-I des (1-3) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 des-1-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the IGF-I des (1-3) in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by a cell proliferation assay using FDC-P1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5ƒâ€”105 units/mg.