Anti-Cyclin B1 (G2- & M-phase Cyclin) Monoclonal Antibody (Clone: V92.1)

It recognizes a protein of 55-62kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Product Specifications

Product Name Alternative

CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Gene Name

CCNB1

Gene ID

891

UniProt

P14635

Host

Mouse

Reactivity

Human, Mouse

Immunogen

Hamster Cyclin B1 protein

Clonality

Monoclonal

Clone

V92.1

Applications

FACS, IF, IP

Components

200 μg/mL of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/mL.

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.

Applications Notes

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunoprecipitation (1-2ug/500ug protein) (precipitates active CDK1/cyclin B1 complexes)