Pacific Blue Conjugated Anti-CD105 / Endoglin Monoclonal Antibody (Clone:MEM-226)

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Product Specifications

Product Name Alternative

ENG||END||Endoglin||HHT1||ORW1

Gene Name

ENG

Gene ID

2022

UniProt

P17813

Host

Mouse

Reactivity

Rat, Human

Immunogen

Recombinant Vaccinia virus containing the human CD105 cDNA.

Target Antigen

CD105

Clonality

Monoclonal

Clone

MEM-226

Applications

FACS

Purification

Purified antibody is conjugated with Pacific Blue NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Components

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Applications Notes

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.