Mouse MMP-2 (30-460) Protein, His Tag (active enzyme)
Mouse MMP-2 (30-460), His Tag (MM2-M52H9) is expressed from human 293 cells (HEK293) . It contains AA Ala 30 - Thr 460 (Accession # P33434-1) . It needs to be activated by agents such as APMA in vitro to have hydrolytic activity.
Product Specifications
Background
Matrix metalloproteinase-2 (MMP-2) is also known as 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A and CLG4A, which belongs to the peptidase M10A family. MMP-2 / CLG4A contains 3 fibronectin type-II domains and 4 hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. MMP-2 cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. MMP2 involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 50.0 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P33434-1
Expression Region
Ala 30 - Thr 460
Host
HEK293
Target
MMP-2
Conjugation
Unconjugated
Tag
C-10xHis
Source
Mouse
Stability
● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 6 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
85%
Format
Liquid
Buffer
50 mM Tris, 150 mM NaCl, pH7.5
Additives
Glycerol
Molecular Weight
50.0 kDa
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
500ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/MM2-M52H9.pdf
Species
Mouse
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
