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Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)

The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.

Product Specifications

Gene Name

N

Gene ID

43740575

UniProt

P0DTC9

Host

Mouse

Reactivity

Human

Immunogen

Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody.

Target Antigen

Nucleocapsid

Clonality

Monoclonal

Clone

ABM1F11.1E1

Applications

ELISA, WB

Purification

Protein G Chromatography

Format

Purified

Components

25 μg in 50 μl/100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Applications Notes

Recommended dilutions: WB: 0.1-1 μg/mL, ELISA: 1 μg/mL. However, this need to be optimized based on the research applications.

Isotype

Mouse IgG1, Kappa

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