CleanCap M6 Cas9 ModTail mRNA (N1MePsU), 1 mg

This mRNA construct, produced viain vitrotranscription (IVT), expresses a version of theStreptococcus pyogenesSF370 CRISPR Associated Protein 9 (Cas9) protein. Cas9 is a key part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In this system, an RNA guide sequence targets a specific site, and the Cas9 protein performs double-stranded DNA cleavage. Key features of this mRNA construct to maximize protein expression include: In addition, our Cas9 mRNA encodes the Cas9 protein with an N and C terminal nuclear localization signal (NLS) . The incorporation of two NLS signals within the mRNA increases the frequency of delivery to the nucleus, thus increasing the rate of DNA cleavage. Additionally, a C terminal HA epitope tag aids in detection, isolation, and purification of the Cas9 protein.