PGA4 Protein (C-His, Human)

Product Specifications

Abbreviation

PGA4

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2, AnaSpec, Catalog # 27114. The specific activity is > 10,000 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)

Function

Met1-Ala388

Molecular Weight

The secreted recombinant human PGA4 consists of 384 amino acids and has a predicted molecular mass of 41.7 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.