IL2RA Protein (C-His, Human)

Product Specifications

Abbreviation

IL2RA

Synonyms

CD25, IDDM10, IL2R, IMD41, p55, TCGFR

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Measured by its ability to inhibit IL2-induced proliferation of Mo7e cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of 5 ng/mL of recombinant human IL2 (Routinely tested) . 2.Loaded Recombinant Human IL2Ra / CD25 Protein (His Tag) (Cat. No. 10165-H08H-UE) on His1K Biosensor, can bind Recombinant Human IL2 Protein (Cat. No. 11848-HNAE2) with an affinity constant of 50.5 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Cys213

Molecular Weight

The recombinant human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation. The molecular weight of this protein is around 32.4 kDa verified by SEC-MALS (Routinely tested) .