PDCD1 Protein (C-His, Cynomolgus)

1.Immobilized PD-1 Protein, Cynomolgus, Recombinant (His Tag) (Cat:90311-C08H) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat:90251-C02H), the EC50 is 16-86 ng/mL (QC tested) . 2.Immobilized PD-1 Protein, Cynomolgus, Recombinant (His Tag) (Cat:90311-C08H) at 2 μg/mL (100 μL/well) can bind Anti-PD1 (Research grade Nivolumab biosimilar), the EC50 is 6-40 ng/mL (Routinely tested) . 3.Immobilized Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 90251-C41H-B) at 5 μg/mL (100 μl/well) on streptavidin precoated (5μg/mL, 100 μL/well) can bind Recombinant Cynomolgus PD-1 Protein (His Tag), HPLC-verified (Cat: 90311-C08H), the EC50 is 187.75-563.55 ng/mL (Routinely tested) . 4.Biotinylated Recombinant Cynomolgus, Rhesus PD-L1/B7-H1 Protein, hFc & AVI Tag (Cat. No. 90251-C41H-B) captured on Protein A chip, can bind Recombinant Cynomolgus PD-1 Protein, His Tag (Cat. No. 90311-C08H) with an affinity constant of 1.200 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

The recombinant cynomolgus PDCD1 consists of 154 amino acids and predicts a molecular mass of 17.4 kDa. As a result of glycosylation, it migrates as an approximately 36.7 kDa band in SDS-PAGE under reducing conditions.