IL18 Protein (N-cleavage, Human)

1.Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 3-36 ng/ml. 2.Loaded Recombinant Human IL-18R beta / IL-18RAP Protein, hFc & AVI Tag (Cat. No. 10176-H41H-B) on ProA Biosensor, can bind Recombinant Human IL-18Rα Protein, His Tag (Cat. No. 11102-H08H) (Premixed with Human IL-18 Protein, Tag Free, Cat. No. 10119-HNCE) with an affinity constant of 7.7nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Recombinant Human IL-18BP Protein, hFc Tag (Cat. No. 10357-H02H) captured on Protein A chip can bind Recombinant Human IL-18 Protein (Cat. No. 10119-HNCE) with an affinity constant of 14.9 pM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 4.Recombinant Human IL-18R beta / IL-18RAP Protein, hFc Tag (Cat. No. 10176-H02H) captured on Protein A chip can bind Recombinant Human IL-18 Protein (Cat. No. 10119-HNCE) (Premixed with Recombinant Human IL-18Rα Protein, His Tag, Cat. No. 11102-H08H) with an affinity constant of 12.85 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Recombinant Human IL18BP Protein (Fc Tag) (Cat: 10357-H02H) captured on Protein A chip, can bind Recombinant Human IL18 / IL-18 Protein (Cat: 10119-HNCE) with an affinity constant of 59.650 pM as determined in an SPR assay (Biacore T200) (Routinely tested) . 6.Recombinant Human IL18 / IL-18 Protein (Cat: 10119-HNCE) immobilized on CM5 chip, can bind Recombinant Human IL-18Rα Protein (ECD, Fc & AVI Tag), Biotinylated (Cat: 11102-H41H-B) with an affinity constant of 71.320 nM as determined in an SPR assay (Biacore T200) (QC tested) .