HGF Protein (Native, Mouse)

Product Specifications

Abbreviation

HGF

Synonyms

C230052L06Rik, Hgf, HGF/SF, NK1, NK2, SF, SF/HGF

Host

HEK 293

Tag

Native

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

1. Immobilized mouse HGF at 10 μg/ml (100 μl/well) can bind mouse MET-Fc (Cat:50622-M02H), EC50 of mouse MET-Fc (Cat:50622-M02H) is 0.05-0.12 μg/ml. 2. Measured by its ability to inhibit TGFß1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.

Function

Met1-Leu728

Molecular Weight

The recombinant mouse HGF comprises 696 amino acids and has a predicted molecular mass of 79.3 kDa. The apparent molecular mass of the protein is approximately 93 kDa in SDS-PAGE under reducing conditions due to glycosylation.