GDNF Protein (N-cleavage, Human)

Product Specifications

Abbreviation

GDNF

Synonyms

ATF, ATF1, ATF2, HFB1-GDNF, HSCR3

Host

HEK 293

Tag

N-cleavage

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Bioactivity

1. Measured in a cell proliferation assay using SH‐SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-3 ng/mL in the presence of Recombinant Human GFR alpha‐1/GDNF R alpha‐1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein (Cat:10561-HNCH) at 2 μg/mL (100 μL/well) can bind Recombinant Human GFRA1 / GFRα1 / GDNFRA Protein (His Tag) (Cat:10330-H08H), the EC50 is 15-48 ng/mL.

Function

Arg83-Ile185

Molecular Weight

The recombinant human GDNF consists of 105 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as approximately 18 KDa band in SDS-PAGE under reducing conditions.