CD274 Protein (C-human IgG1-Fc, Mouse)

1.Measured by its binding ability in a functional ELISA. 2.Immobilized Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag) (Cat: 50010-M02H) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse PD-1 Protein (Fc & AVI Tag), Biotinylated (Cat: 50124-M41H-B), the EC50 is 200-750 ng/mL. (QC tested) 3.Immobilized Recombinant Mouse PD-1 Protein (His & AVI Tag), Biotinylated (Cat: 50124-M49H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Mouse PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag) (Cat: 50010-M02H), the EC50 is 4-12 ng/mL. (Routinely tested) 4.Loaded Recombinant Mouse PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 50010-M02H) on ProA Biosensor, can bind Recombinant Mouse PD-1 Protein, His Tag (Cat. No. 50124-M08H) with an affinity constant of 3.6 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant mouse Cd274 consists 459 amino acids and predicts a molecular mass of 51.6 kDa. As a result of glycosylation, it migrates as an approximately 74.3 kDa band in SDS-PAGE under reducing conditions.