Spike Protein (C-His, SARS)

Product Specifications

Abbreviation

Spike

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) (10108-H05H) at 2μg/mL (100μL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) (40150-V08B1), the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag) (40150-V08B1) is 400-850 ng/mL.

Function

Met1-Arg667

Molecular Weight

The recombinant S1 subunit of SARS-CoV (isolate:WH20) spike comprises 665 amino acids and has a predicted molecular mass of 74.4 kDa. The apparent molecular mass of the protein is approximately 85.8 kDa in SDS-PAGE under reducing conditions.