MUC16 Protein (C-His, Human)

Product Specifications

Abbreviation

MUC16

Synonyms

CA-125, CA125, FLJ14303

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Recombinant Human MSLN / Mesothelin Protein (Cat:13128-HNCH) at 2 μg/ml (100 μl/well) can bind Recombinant Human CA125 Protein (ECD, His Tag) (Cat:30081-H08H), the EC50 is 6-18 ng/mL (QC tested) . 2.Recombinant Human MSLN / Mesothelin Protein (His Tag) (Cat: 13128-H08H1) immobilized on CM5 chip, can bind Recombinant Human CA125 Protein (ECD, His Tag) (Cat: 30081-H08H) with an affinity constant of 3.710 μM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Pro13810-Pro14451

Molecular Weight

The recombinant human MUC16 consists of 653 amino acids and predicts a molecular mass of 73.27 kDa. It migrates as an approximately 101.25 kDa band in SDS-PAGE under reducing conditions.