INSR Protein (C-His, Human)

Product Specifications

Abbreviation

INSR

Synonyms

CD220, HHF5

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Recombinant Human Insulin Receptor Protein (ECD, long isoform), His Tag (Cat. No. 11081-H08H) captured on CM5 chip via Anti-His antibody, can bind Insulin with an affinity constant of 352 nM as determined in an SPR assay (Biacore T200) (QC tested) .

Function

Met1-Lys956

Molecular Weight

The secreted recombinant human INSR isoform long consists of 940 amino acids and has a predicted molecular mass of 107 (83+24) kDa. As a result of glycosylation, the apparent molecular mass of human INSR is approximately 110-125 kDa & 40-45 kDa, corresponding to the α subunit and the ECD of β subunit respectively in SDS-PAGE under reducing conditions.