CD19 Protein (C-His, Human)

1.Immobilized Recombinant Human CD19 Protein (ECD, His Tag) (Cat: 11880-H08W1) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti- CD19 Antibody, Human IgG1, the EC50 is 4-15 ng/mL. (Routinely tested) 2.Loaded Biotinylated Inebilizumab on SA Biosensor, can bind Recombinant Human CD19 Protein, His Tag, High activity (Cat. No. 11880-H08W1) with an affinity constant of 9.58 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Monoclonal Anti- CD19 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD19 Protein (ECD, His Tag) (Cat: 11880-H08W1), the EC50 is 15-50 ng/mL. (QC tested) 4.Inebilizumab captured on Protein A chip can bind Recombinant Human CD19 Protein, His Tag (Cat. No. 11880-H08W1) with an affinity constant of 0.498 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 5.Tafasitamab captured on Protein A chip can bind Recombinant Human CD19 Protein, His Tag (Cat. No. 11880-H08W1) with an affinity constant of 5.088 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 6.Coltuximab captured on Protein A chip can bind Recombinant Human CD19 Protein, His Tag (Cat. No. 11880-H08W1) with an affinity constant of 3.310 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant Human CD19 consisting of 283 amino acids and has a calculated molecular mass of 31.56 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45.3 kDa protein in SDS-PAGE under reducing conditions.