Anti-Neurofilament light polypeptide (NF-L) Antibody (7D1)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is igg. It is validated for use in IF, ICC, WB.

Product Specifications

Background

Neurofilaments are composed of three intermediate filament proteins: light (NF-L ~68 kDa), medium (NF-M ~160 kDa) and heavy (NF-H ~200 kDa), found specifically in neurons, which are involved in the maintenance of the neuronal caliber. Neurofilament light (NF68 or NF-L) is the most abundant of the three proteins. (Ref: uniprot.org)

Short Description

Synonyms

NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL

UniProt

P07196

Reactivity

Bovine, Horse, Human, Mouse, Pig, and Rat

Immunogen

The antibody has been made against a preparation of recombinant full length human NF-L; native protein purified from pig spinal cord. The antibody binds NF-L from a variety of species including human, rat and mouse.

Target

Neurofilament light polypeptide (NF-L)

Clonality

Monoclonal

Clone

7D1

Conjugation

Unconjugated

Applications

IF, ICC, WB

Format

IgG

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 50 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

Notes

Protein G purified

WB Dilution

1:5000-1:10000

Specificity

Species cross-reactivity includes human, rat, mouse, cow, pig and horse.

ICC Dilution

1:100-1:500

Host or Source

Mouse

Physical Properties

Lyophilized

Isotype

IgG2b

Immunogen Species

Human

Curated Selection

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