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Anti-Tyrosine Hydroxylase (TH) Antibody

Our Anti-Tyrosine Hydroxylase (TH) ; Tyrosine 3-monooxyegnase chicken polyclonal primary antibody detects human, mouse, and rat Tyrosine Hydroxylase (TH) ; Tyrosine 3-monooxyegnase, and is igy. It is validated for use in FC, ICC, IHC-Frozen, WB.

Product Specifications

Background

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa. In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity) . Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity) . (Ref: uniprot.org)

Short Description

Our Anti-Tyrosine Hydroxylase (TH) ; Tyrosine 3-monooxyegnase chicken polyclonal primary antibody detects human, mouse, and rat Tyrosine Hydroxylase (TH) ; Tyrosine 3-monooxyegnase, and is IgY. It is validated for use in FC, ICC, IHC-Frozen, WB.

Synonyms

TH; T-H; tyrosine 3-monooxygenase; monooxygenase; oxidoreductase

UniProt

P07101

Reactivity

Human, Mouse, and Rat

Immunogen

The antibody has been made against the full length recombinant human TH (524 amino acid sequence) expressed in and purified from E. Coli

Target

Tyrosine Hydroxylase (TH) ; Tyrosine 3-monooxyegnase

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

FC, ICC, IHC-Frozen, WB

Format

IgY

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Precautions

For research use only.

Shipping Conditions

25°C (ambient)

Storage Conditions

Spin vial briefly before opening. Reconstitute with 100 μL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

IHC Dilution

1:100-1:500

WB Dilution

1:5000-1:10000

Specificity

Human. Species cross-reactivity includes rat and mouse.

ICC Dilution

1:100-1:500

Host or Source

Chicken

Physical Properties

Lyophilized

Isotype

IgY

Immunogen Species

Human

Curated Selection

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