Anti-Mouse ICAM-1 (BE29G1) In Vivo Antibody - Low Endotoxin
Product Specifications
Background
Intercellular adhesion molecule-1 (ICAM-1) is one of an important cell adhesion molecules (CAMs) family transmembrane glycoprotein and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. ICAM-1 is a marker of endothelial dysfunction leading to damaging vascular disorders, in umbilical and placental vascular tissue of gestational pregnancies
Product Name Alternative
Intercellular adhesion molecule 1, Icam1, MALA-2, MyD10, CD54
UniProt
P13597
Host
Rat
Reactivity
Mouse
Immunogen
Purified Recombinant Mouse ICAM-1 (>98%)
Target
ICAM-1
Isotype
IgG2a
Clone
BE29G1
Applications
Immunoprecipitation, Western Blot, Blocking
Purification Method
This monoclonal antibody was purified using Protein G
Concentration
0.5 mg/mL
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Shipping Conditions
Blue ice
Storage Conditions
Anti-ICAM-1 In Vivo Antibody - Low Endotoxin (BE29G1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Specificity
Anti-ICAM-1 In Vivo Antibody - Low Endotoxin (BE29G1) recognizes the (Mr 95 kD) cell surface glycoprotein known as intracellular adhesion molecule ICAM-1. ICAM-1 is the receptor for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) .
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The CD54 antigen is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. It has been demonstrated that ICAM-1 enhances T-cell activation.
Aggregation
Aggregation level ≤ 5%
Applications Notes
Blocking: This antibody blocks the binding LFA-1 (CD11a/CD18) to ICAM-1. The antibody is suitable for studies of inflammation, lymphocyte differentiation, and lymphocyte activation. Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244)
Antibodies Against Same Target
Anti-ICAM-1 In Vivo Antibody - Low Endotoxin [15.2] (ICH1027)
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