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Human PP1 Catalytic Recombinant Protein

Human PP1 Catalytic Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-330aa. Application (s) : SDS-PAGE, Enzyme Activity.

Product Specifications

Synonyms

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A

Gene Name

PPP1CA

UniProt

P62136

Tag

His-Tag

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Enzymes and Proteases

Purification

> 80% by SDS-PAGE

Concentration

0.2mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH7.5 using 10mM of substrate.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

39.7 kDa (350aa) confirmed by MALDI-TOF

Contents

50mM Tris-HCl buffer (pH 8.5) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2, 50%glycerol

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