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Human Aldose reductase Recombinant Protein

Human Aldose reductase Recombinant Protein expressed in E. coli (non-tagged) . Sequence domain: 1-316aa. Application (s) : SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

Product Specifications

Synonyms

Aldo-keto reductase family1 member B1, AKR1B1, ADR, ALDR1, ALR2, AR, Aldose reductase, AKR1B 1, Aldehyde reductase, aldr 1, aldr-1, Lii5-2 CTCL tumor antigen, low Km aldose reductase

Gene Name

AKR1B1

UniProt

P15121

Tag

Non-Tagged

Source

E. coli

Applications

SDS-PAGE, Enzyme Activity

Field of Research

Redox Proteins

Purification

> 95% by SDS-PAGE

Concentration

1mg/mL (determined by Bradford assay)

Bioactivity

Specific activity is >800 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction of 1.0 pmole DL-glyceraldehyde in the presence of NADPH per minute at pH7.0 at 37°C.

Form

Liquid

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)

Observed Molecular Weight

35.8 kDa (316aa) confirmed by MALDI-TOF

Contents

20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

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