Anti-USP25 Antibody
Boster Bio Anti-USP25 Antibody (Catalog # M06182) . Tested in WB application (s) . This antibody reacts with Human.
Product Specifications
Background
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Synonyms
Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21
Gene Name
USP25
UniProt
Q9UHP3
Host
Mouse
Reactivity
Human
Immunogen
This USP25 antibody is generated from a mouse immunized with a recombinant protein from human USP25.
Clonality
Monoclonal
Clone
Clone: 1277CT376.106.171
Tissue Specificity
Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific
Applications
WB
Field of Research
Cell Biology
Purification
This antibody is purified through a protein G column, followed by dialysis against PBS.
Function
Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates. Hydrolyzes both 'Lys-48'- and 'Lys-63'-linked tetraubiquitin chains.
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Observed Molecular Weight
122218 Da
Applications Notes
WB: 1:1000
Subcellular Location
Cytoplasm
Isotype
IgG1, κ
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
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