Human EPF/HSP10 ELISA Kit PicoKine®

Heat shock 10 kDa protein 1 (Hsp10), also known as chaperonin 10 (cpn10) or early-pregnancy factor (EPF), is a protein that in humans is encoded by the HSPE1 gene. This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 112 125 101 112 4.01 3.6% Sample 2 456 496 485 422 465 22.77 4.9% Sample 3 1755 1896 1744 1963 1840 93.81 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 112 125 101 112 4.01 3.6% Sample 1 108 112 125 101 112 4.01 3.6% Sample 2 456 496 485 422 465 22.77 4.9% Sample 2 456 496 485 422 465 22.77 4.9% Sample 3 1755 1896 1744 1963 1840 93.81 5.1% Sample 3 1755 1896 1744 1963 1840 93.81 5.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 456 1755 115 469 1896 Mean (pg/ml) 108 456 1755 115 469 1896 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 Standard deviation 5.94 22.34 107.06 5.52 25.8 81.53 CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3% CV (%) 5.5% 4.9% 6.1% 4.8% 5.5% 4.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48 O.D. 0.01 0.08 0.154 0.26 0.504 0.929 1.649 2.48

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.