Human Langerin/CD207 ELISA Kit PicoKine®

Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. The protein encoded by this gene is expressed only in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. It is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Mutations in this gene result in Birbeck granules deficiency or loss of sugar binding activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 195 198 205 193 10.96 5.6% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 3 5456 6236 5869 57415 5826 262.15 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 185 195 198 205 193 10.96 5.6% Sample 1 185 195 198 205 193 10.96 5.6% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 2 1366 1296 1142 1223 1257 74.15 5.9% Sample 3 5456 6236 5869 57415 5826 262.15 4.5% Sample 3 5456 6236 5869 57415 5826 262.15 4.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 185 1366 5456 183 1469 5812 Mean (pg/ml) 185 1366 5456 183 1469 5812 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 Standard deviation 8.88 88.79 349.18 12.63 113.11 412.65 CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1% CV (%) 4.8% 6.5% 6.4% 6.9% 7.7% 7.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044 O.D. 0.025 0.094 0.145 0.252 0.43 0.759 1.388 2.044

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.