Human CD157/BST1 ELISA Kit PicoKine®

Bst1 (Bone marrow stromal cell antigen 1, ADP-ribosyl cyclase 2, CD157) is an enzyme that in humans is encoded by the BST1 gene. Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 3 5140 5265 5333 5441 5295 232.97 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 1 1255 1354 1264 1280 1288 37.36 2.9% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 2 2656 2740 2895 2874 2791 142.35 5.1% Sample 3 5140 5265 5333 5441 5295 232.97 4.4% Sample 3 5140 5265 5333 5441 5295 232.97 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Mean (pg/ml) 1255 2656 5140 1420 2862 5655 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 Standard deviation 72.79 108.9 185.04 95.14 148.82 277.1 CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9% CV (%) 5.8% 4.1% 3.6% 6.7% 5.2% 4.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153 O.D. 0.029 0.115 0.173 0.31 0.537 0.976 1.555 2.153

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.