Human ACHE ELISA Kit PicoKine®

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 39 59 49 1.61 3.3% Sample 2 163 188 174 150 169 7.76 4.6% Sample 3 278 312 333 329 313 18.47 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 46 51 39 59 49 1.61 3.3% Sample 1 46 51 39 59 49 1.61 3.3% Sample 2 163 188 174 150 169 7.76 4.6% Sample 2 163 188 174 150 169 7.76 4.6% Sample 3 278 312 333 329 313 18.47 5.9% Sample 3 278 312 333 329 313 18.47 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 163 278 52 178 305 Standard deviation 2.07 7.66 9.17 2.7 10.32 15.86 CV (%) 4.5% 4.7% 3.3% 5.2% 5.8% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 46 163 278 52 178 305 Standard deviation 2.07 7.66 9.17 2.7 10.32 15.86 CV (%) 4.5% 4.7% 3.3% 5.2% 5.8% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 46 163 278 52 178 305 Mean (pg/ml) 46 163 278 52 178 305 Standard deviation 2.07 7.66 9.17 2.7 10.32 15.86 Standard deviation 2.07 7.66 9.17 2.7 10.32 15.86 CV (%) 4.5% 4.7% 3.3% 5.2% 5.8% 5.2% CV (%) 4.5% 4.7% 3.3% 5.2% 5.8% 5.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.093 0.163 0.321 0.639 1.026 1.628 2.072 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.093 0.163 0.321 0.639 1.026 1.628 2.072 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.03 0.093 0.163 0.321 0.639 1.026 1.628 2.072 O.D. 0.03 0.093 0.163 0.321 0.639 1.026 1.628 2.072

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.