Human LILRB5/CD85c/LIR-8 ELISA Kit PicoKine®

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) . Several other LIR subfamily B receptors are expressed on immune cells where they bind to MHC class I molecules on antigen-presenting cells and inhibit stimulation of an immune response. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 316 297 305 322 310 19.22 5.2% Sample 2 1622 1529 1646 1531 1582 96.50 6.1% Sample 3 4807 4976 4716 4921 4855 398.11 8.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 316 297 305 322 310 19.22 5.2% Sample 1 316 297 305 322 310 19.22 5.2% Sample 2 1622 1529 1646 1531 1582 96.50 6.1% Sample 2 1622 1529 1646 1531 1582 96.50 6.1% Sample 3 4807 4976 4716 4921 4855 398.11 8.2% Sample 3 4807 4976 4716 4921 4855 398.11 8.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 316 1622 4807 329 1719 5032 Standard deviation 18.33 72.99 293.23 25.33 147.83 367.34 CV (%) 5.8% 4.5% 6.1% 7.7% 8.6% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 316 1622 4807 329 1719 5032 Standard deviation 18.33 72.99 293.23 25.33 147.83 367.34 CV (%) 5.8% 4.5% 6.1% 7.7% 8.6% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 316 1622 4807 329 1719 5032 Mean (pg/ml) 316 1622 4807 329 1719 5032 Standard deviation 18.33 72.99 293.23 25.33 147.83 367.34 Standard deviation 18.33 72.99 293.23 25.33 147.83 367.34 CV (%) 5.8% 4.5% 6.1% 7.7% 8.6% 7.3% CV (%) 5.8% 4.5% 6.1% 7.7% 8.6% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.157 0.208 0.314 0.582 0.924 1.437 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.157 0.208 0.314 0.582 0.924 1.437 2.013 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.063 0.157 0.208 0.314 0.582 0.924 1.437 2.013 O.D. 0.063 0.157 0.208 0.314 0.582 0.924 1.437 2.013

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.