Human DNER ELISA Kit PicoKine®

Human DNER ELISA Kit PicoKine® (96 Tests) . Quantitate Human DNER in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 30pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 2q36.3. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 76 117 106 94 3.65 3.9% Sample 2 174 203 247 148 193 11.77 6.1% Sample 3 1710 1611 1200 1578 1525 95.6 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 75 76 117 106 94 3.65 3.9% Sample 1 75 76 117 106 94 3.65 3.9% Sample 2 174 203 247 148 193 11.77 6.1% Sample 2 174 203 247 148 193 11.77 6.1% Sample 3 1710 1611 1200 1578 1525 95.6 6.3% Sample 3 1710 1611 1200 1578 1525 95.6 6.3%

Synonyms

Delta and Notch-like epidermal growth factor-related receptor; DNER; BET; UNQ262; PRO299

Gene Name

DNER

Gene ID

92737

UniProt

Q8NFT8

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N35-Y640

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in brain, spinal cord and adrenal gland.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 194 1626 78 127 1130 Mean (pg/ml) 111 194 1626 78 127 1130 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 Standard deviation 4 4.15 38.86 4.95 4.15 36.5 CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2% CV (%) 3.6% 2.1% 2.4% 6.3% 3.3% 3.2%

Sensitivity

<30 pg/mL

Function

Activator of the NOTCH1 pathway. May mediate neuron-glia interaction during astrocytogenesis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human DNER

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Delta/notch like EGF repeat containing

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474 O.D. 0.008 0.092 0.159 0.308 0.626 1.104 1.872 2.474

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.