Human Nectin-2/CD112 ELISA Kit PicoKine®

Poliovirus receptor-related 2 (PVRL2), also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), is a human plasma membrane glycoprotein. It is mapped to 19q13.32. This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 194 202 199 201 6.18 3.0% Sample 2 1599 1649 1794 1735 1694 75.38 4.4% Sample 3 5167 5008 5074 5608 5214 234.24 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 211 194 202 199 201 6.18 3.0% Sample 1 211 194 202 199 201 6.18 3.0% Sample 2 1599 1649 1794 1735 1694 75.38 4.4% Sample 2 1599 1649 1794 1735 1694 75.38 4.4% Sample 3 5167 5008 5074 5608 5214 234.24 4.4% Sample 3 5167 5008 5074 5608 5214 234.24 4.4%

Nectin-2; Herpes virus entry mediator B; Herpesvirus entry mediator B; HveB; Nectin cell adhesion molecule 2; Poliovirus receptor-related protein 2; CD112; NECTIN2; HVEB; PRR2; PVRL2

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 1599 5167 264 1794 5274 Standard deviation 9.92 84.75 289.35 17.69 91.49 253.15 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 211 1599 5167 264 1794 5274 Standard deviation 9.92 84.75 289.35 17.69 91.49 253.15 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 211 1599 5167 264 1794 5274 Mean (pg/ml) 211 1599 5167 264 1794 5274 Standard deviation 9.92 84.75 289.35 17.69 91.49 253.15 Standard deviation 9.92 84.75 289.35 17.69 91.49 253.15 CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8% CV (%) 4.7% 5.3% 5.6% 6.7% 5.1% 4.8%

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive. Probable cell adhesion protein. (Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.