beta Actin Recombinant Rabbit Monoclonal Antibody [PSH03-63]
Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
Beta-actin
Swiss Prot
P60709 Human, P60710 Mouse, P60711 Rat
Cellular Locus
Cytoskeleton.
Host
Rabbit
Species Reactivity
Human,Mouse,Rat,Rabbit,Goat,Zebrafish,Dog,Monkey,Pig,Chicken,Cow,Hamster,Green monkey
Immunogen
Synthetic peptide within N-terminal residues of β-Actin.
Isotype
IgG
Clone
PSH03-63
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, FC, IP
Positive Control
Concentration
1 mg/mL
Dilution
WB: 1:20,000-1:640,000 ;IF-Cell: 1:100-1:250 ;FC: 1:1,000 ;IP: 1-2μg/sample
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 42 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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