Human P4HB ELISA Kit PicoKine®

Human P4HB ELISA Kit PicoKine® (96 Tests) . Quantitate Human P4HB in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. It is mapped to 17q25.3. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 155 171 167 157 162 6.68 4.1% Sample 2 785 728 688 729 732 34.52 4.7% Sample 3 2454 2351 2283 2430 2379 67.50 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 155 171 167 157 162 6.68 4.1% Sample 1 155 171 167 157 162 6.68 4.1% Sample 2 785 728 688 729 732 34.52 4.7% Sample 2 785 728 688 729 732 34.52 4.7% Sample 3 2454 2351 2283 2430 2379 67.50 2.8% Sample 3 2454 2351 2283 2430 2379 67.50 2.8%

Synonyms

Protein disulfide-isomerase; PDI; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; P4HB; ERBA2L; PDI; PDIA1; PO4DB

Gene Name

P4HB

Gene ID

5034

UniProt

P07237

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D18-L508

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Metabolism, Metabolism of Lipids and Lipoproteins, Pathways and Processes, Protein Trafficking, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.8 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 155 785 2454 177 790 2763 Standard deviation 8.84 55.74 107.98 10.09 48.19 212.75 CV (%) 5.7% 7.1% 4.4% 5.7% 6.1% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 155 785 2454 177 790 2763 Standard deviation 8.84 55.74 107.98 10.09 48.19 212.75 CV (%) 5.7% 7.1% 4.4% 5.7% 6.1% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 155 785 2454 177 790 2763 Mean (pg/ml) 155 785 2454 177 790 2763 Standard deviation 8.84 55.74 107.98 10.09 48.19 212.75 Standard deviation 8.84 55.74 107.98 10.09 48.19 212.75 CV (%) 5.7% 7.1% 4.4% 5.7% 6.1% 7.7% CV (%) 5.7% 7.1% 4.4% 5.7% 6.1% 7.7%

Sensitivity

<15 pg/mL

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modificatio

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human P4HB

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Prolyl 4-hydroxylase subunit beta

Subcellular Location

Endoplasmic reticulum, Endoplasmic reticulum lumen, Cell membrane, Peripheral membrane protein, Melanosome

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.025 0.16 0.263 0.491 0.852 1.29 1.878 2.305 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.025 0.16 0.263 0.491 0.852 1.29 1.878 2.305 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.025 0.16 0.263 0.491 0.852 1.29 1.878 2.305 O.D. 0.025 0.16 0.263 0.491 0.852 1.29 1.878 2.305

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.