Mouse PDGF-BB ELISA Kit PicoKine®

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 172 178 169 167 10.88 6.5% Sample . 2 683 696 665 663 676 13.57 2.0%% Sample 3 1203 1369 1343 1427 1335 82.32 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 172 178 169 167 10.88 6.5% Sample 1 149 172 178 169 167 10.88 6.5% Sample . 2 683 696 665 663 676 13.57 2.0%% Sample . 2 683 696 665 663 676 13.57 2.0%% Sample 3 1203 1369 1343 1427 1335 82.32 6.1% Sample 3 1203 1369 1343 1427 1335 82.32 6.1%

Epidermal growth factor-like protein 7; EGF-like protein 7; Multiple epidermal growth factor-like domains protein 7; Multiple EGF-like domains protein 7; NOTCH4-like protein; Vascular endothelial statin; VE-statin; Zneu1; Egfl7; Megf7

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Growth Factors, Growth Factors/Hormones, Immunology, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Pathways and Processes, Platelets, Response To Hypoxia, Secreted Molecules, Serum Proteins, Signal Transduction, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 683 1203 150 750 1331 Standard deviation 6.7 48.49 56.54 7.95 43.5 82.5 CV (%) 4.5% 7.1% 4.7% 5.3% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 683 1203 150 750 1331 Standard deviation 6.7 48.49 56.54 7.95 43.5 82.5 CV (%) 4.5% 7.1% 4.7% 5.3% 5.8% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 149 683 1203 150 750 1331 Mean (pg/ml) 149 683 1203 150 750 1331 Standard deviation 6.7 48.49 56.54 7.95 43.5 82.5 Standard deviation 6.7 48.49 56.54 7.95 43.5 82.5 CV (%) 4.5% 7.1% 4.7% 5.3% 5.8% 6.2% CV (%) 4.5% 7.1% 4.7% 5.3% 5.8% 6.2%

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF) -BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.071 0.124 0.193 0.375 0.698 1.263 2.076 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.071 0.124 0.193 0.375 0.698 1.263 2.076 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.026 0.071 0.124 0.193 0.375 0.698 1.263 2.076 O.D. 0.026 0.071 0.124 0.193 0.375 0.698 1.263 2.076

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.