Human Dystroglycan ELISA Kit PicoKine®

Human Dystroglycan ELISA Kit PicoKine® (96 Tests) . Quantitate Human DAG1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dystrophin, also known as DMD, is a rod-shaped cytoplasmic protein, and a vital part of a protein complex that connects the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. It is mapped to Xp21.2-p21.1. This complex is variously known as the costamere or thedystrophin-associated protein complex. Many muscle proteins, such as alpha-dystrobrevin, syncoilin, synemin, sarcoglycan, dystroglycan, and sarcospan, colocalize with dystrophin at the costamere. Dystrophin is a protein located between the sarcolemma and the outermost layer of myofilaments in the muscle fiber (myofiber) . It is a cohesive protein, linking actin filaments to another support protein that resides on the inside surface of each muscle fiber’s plasma membrane (sarcolemma) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 331 321 338 325 10.55 3.2% Sample 2 1339 1116 1160 1311 1231 95.3 7.7% Sample 3 5007 5679 5616 4840 5285 367.45 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 331 321 338 325 10.55 3.2% Sample 1 310 331 321 338 325 10.55 3.2% Sample 2 1339 1116 1160 1311 1231 95.3 7.7% Sample 2 1339 1116 1160 1311 1231 95.3 7.7% Sample 3 5007 5679 5616 4840 5285 367.45 6.9% Sample 3 5007 5679 5616 4840 5285 367.45 6.9%

Synonyms

Dystroglycan; Dystrophin-associated glycoprotein 1; Alpha-dystroglycan; Alpha-DG; Beta-dystroglycan; Beta-DG; DAG1

Gene Name

DAG1

Gene ID

1605

UniProt

Q14118

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H30-G653

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.

Applications

ELISA

Field of Research

Actin Binding Proteins, Actin, etc., Cytoskeleton, Cytoskeleton/ECM, Microfilaments, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1339 5007 327 1273 4624 Standard deviation 19.53 62.93 285.39 22.56 78.92 346.8 CV (%) 6.2%% 4.7% 5.7% 6.9% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1339 5007 327 1273 4624 Standard deviation 19.53 62.93 285.39 22.56 78.92 346.8 CV (%) 6.2%% 4.7% 5.7% 6.9% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1339 5007 327 1273 4624 Mean (pg/ml) 310 1339 5007 327 1273 4624 Standard deviation 19.53 62.93 285.39 22.56 78.92 346.8 Standard deviation 19.53 62.93 285.39 22.56 78.92 346.8 CV (%) 6.2%% 4.7% 5.7% 6.9% 6.2% 7.5% CV (%) 6.2%% 4.7% 5.7% 6.9% 6.2% 7.5%

Sensitivity

<10 pg/mL

Function

The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Dystroglycan

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Dystroglycan 1

Subcellular Location

Alpha-dystroglycan: Secreted, extracellular space.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.085 0.139 0.261 0.478 0.854 1.582 2.237 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.085 0.139 0.261 0.478 0.854 1.582 2.237 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.085 0.139 0.261 0.478 0.854 1.582 2.237 O.D. 0.033 0.085 0.139 0.261 0.478 0.854 1.582 2.237

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.