Mouse OSM/Oncostatin M ELISA Kit PicoKine®

OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 57 59 53 57 2.95 5.1% Sample 2 260 248 220 250 244 14.85 6% Sample 3 822 851 792 912 844 44.33 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 57 59 53 57 2.95 5.1% Sample 1 61 57 59 53 57 2.95 5.1% Sample 2 260 248 220 250 244 14.85 6% Sample 2 260 248 220 250 244 14.85 6% Sample 3 822 851 792 912 844 44.33 5.2% Sample 3 822 851 792 912 844 44.33 5.2%

Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 260 822 59 280 850 Mean (pg/ml) 61 260 822 59 280 850 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 Standard deviation 4.51 16.64 36.16 5.31 22.4 52.7 CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2% CV (%) 7.4% 6.4% 4.4% 9% 8% 6.2%

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity) . Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST) . Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807 O.D. 0.029 0.098 0.157 0.26 0.474 0.924 1.63 2.807

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.