Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF) . IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 37 35 34 2.27 6.6% Sample 2 220 228 267 256 242 19.35 7.9% Sample 3 453 402 397 434 421 23.07 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 36 31 37 35 34 2.27 6.6% Sample 1 36 31 37 35 34 2.27 6.6% Sample 2 220 228 267 256 242 19.35 7.9% Sample 2 220 228 267 256 242 19.35 7.9% Sample 3 453 402 397 434 421 23.07 5.4% Sample 3 453 402 397 434 421 23.07 5.4%

Interleukin-5; IL-5; B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; T-cell replacing factor; TRF; Il5; Il-5

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 36 220 453 34 214 478 Mean (pg/ml) 36 220 453 34 214 478 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 Standard deviation 2.52 9.24 21.29 2.58 10.7 30.59 CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4% CV (%) 7% 4.2% 4.7% 7.6% 5% 6.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952 O.D. 0.027 0.097 0.162 0.265 0.471 0.803 1.333 1.952

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.