Human IL-17E/IL-25 ELISA Kit PicoKine®

Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 151 150 157 155 4.84 3.1% Sample 2 689 601 681 631 650 36.2 5.5% Sample 3 1026 1036 1126 1127 1078 47.88 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 162 151 150 157 155 4.84 3.1% Sample 1 162 151 150 157 155 4.84 3.1% Sample 2 689 601 681 631 650 36.2 5.5% Sample 2 689 601 681 631 650 36.2 5.5% Sample 3 1026 1036 1126 1127 1078 47.88 4.4% Sample 3 1026 1036 1126 1127 1078 47.88 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 162 689 1026 168 748 1001 Mean (pg/ml) 162 689 1026 168 748 1001 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 Standard deviation 8.91 42.02 50.27 10.92 57.59 53.05 CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3% CV (%) 5.5% 6.1% 4.9% 6.5% 7.7% 5.3%

Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102 O.D. 0.05 0.086 0.143 0.269 0.504 0.925 1.614 2.102

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.