Human Cathepsin E ELISA Kit PicoKine®

Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 24 25 26 25 1.11 4.4% Sample 2 63 61 69 71 66 4.12 6.2% Sample 3 416 414 399 412 410 6.64 1.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 24 25 26 25 1.11 4.4% Sample 1 27 24 25 26 25 1.11 4.4% Sample 2 63 61 69 71 66 4.12 6.2% Sample 2 63 61 69 71 66 4.12 6.2% Sample 3 416 414 399 412 410 6.64 1.6% Sample 3 416 414 399 412 410 6.64 1.6%

Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes. .

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 63 416 30 82 435 Standard deviation 1.30 3.59 19.14 1.53 4.84 27.84 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 63 416 30 82 435 Standard deviation 1.30 3.59 19.14 1.53 4.84 27.84 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 63 416 30 82 435 Mean (pg/ml) 27 63 416 30 82 435 Standard deviation 1.30 3.59 19.14 1.53 4.84 27.84 Standard deviation 1.30 3.59 19.14 1.53 4.84 27.84 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.099 0.14 0.188 0.318 0.612 1.13 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.099 0.14 0.188 0.318 0.612 1.13 1.84 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.053 0.099 0.14 0.188 0.318 0.612 1.13 1.84 O.D. 0.053 0.099 0.14 0.188 0.318 0.612 1.13 1.84

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.