Mouse CD22/Siglec 2 ELISA Kit PicoKine®

CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors (BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 242 251 262 248 8.69 3.5% Sample 2 2070 1778 1779 2120 1936 159.23 8.2% Sample 3 4058 4096 4754 4460 4342 284.97 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 242 251 262 248 8.69 3.5% Sample 1 240 242 251 262 248 8.69 3.5% Sample 2 2070 1778 1779 2120 1936 159.23 8.2% Sample 2 2070 1778 1779 2120 1936 159.23 8.2% Sample 3 4058 4096 4754 4460 4342 284.97 6.5% Sample 3 4058 4096 4754 4460 4342 284.97 6.5%

B-cell receptor CD22; B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD22; Cd22; Lyb-8, Siglec2

Adaptive Immunity, B Cells, Cancer, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 2070 4058 246 1914 3971 Standard deviation 16.56 124.2 182.61 18.94 132.06 218.4 CV (%) 6.9% 6% 4.5% 7.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 2070 4058 246 1914 3971 Standard deviation 16.56 124.2 182.61 18.94 132.06 218.4 CV (%) 6.9% 6% 4.5% 7.7% 6.9% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 240 2070 4058 246 1914 3971 Mean (pg/ml) 240 2070 4058 246 1914 3971 Standard deviation 16.56 124.2 182.61 18.94 132.06 218.4 Standard deviation 16.56 124.2 182.61 18.94 132.06 218.4 CV (%) 6.9% 6% 4.5% 7.7% 6.9% 5.5% CV (%) 6.9% 6% 4.5% 7.7% 6.9% 5.5%

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.07 0.099 0.209 0.335 0.713 1.311 1.976 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.07 0.099 0.209 0.335 0.713 1.311 1.976 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.07 0.099 0.209 0.335 0.713 1.311 1.976 O.D. 0.008 0.07 0.099 0.209 0.335 0.713 1.311 1.976

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.